Coding

Part:BBa_K4905001:Design

Designed by: Merel van den Bosch   Group: iGEM23_TU-Eindhoven   (2023-06-13)


Elastin-Like polypeptide (VPGIG)[60]-(VPGAG)3(VPGGG)2[12]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1890
    Illegal XbaI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1890
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1890
    Illegal XhoI site found at 1907
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1890
    Illegal XbaI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1890
    Illegal XbaI site found at 7
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 244
    Illegal NgoMIV site found at 334
    Illegal NgoMIV site found at 514
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The chosen amino acids greatly influence the properties of the ELP.


Source

https://en.wikipedia.org/wiki/Elastin-like_polypeptides

References